A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective.

South Africa is home to numerous indigenous and locally developed sheep (Nguni Pedi, Zulu, and Namaqua Afrikaner, Afrino, Africander, Bezuidenhout Africander, Damara, Dorper, Döhne Merino, Meat Master, South African Merino, South African Mutton Merino, Van Rooy, and Dorper), goat (SA veld, Tankwa, Imbuzi, Bantu, Boer, and Savanna) and cattle (Afrigus, Afrikaner, Bolowana, Bonsmara, Bovelder, Drakensberger, South African Angus, South African Dairy Swiss, South African Friesland, South African Red, and Veld Master) animals. These breeds require less veterinary service, feed, management efforts, provide income to rural and or poor owners. However, most of them are under extinction risks and some with unknown status hence, require immediate conservation intervention. To allow faster genetic progress on the endangered animals, it is important to generate productive animals while reducing wastages and this can be achieved through sex-sorted semen. Therefore, this systematic review is aimed to evaluate the prospects of X and Y-sexed semen in ruminant livestock and some solutions that can be used to address poor sex-sorted semen and its fertility. This review was incorporated through gathering and assessing relevant articles and through the data from the DAD-IS database. The keywords that were used to search articles online were pre-gender selection, indigenous ecotypes, fertility, flow cytometry, artificial insemination, conservation, and improving sexed semen. Following a careful review of all articles, PRISMA guidelines were used to find the articles that are suitable to address the aim of this review. Sex-sorted semen is a recently introduced technology gaining more attention from researchers particularly, in the conservation programs. Preselection of semen based on the sex chromosomes (X- and or Y-bearing chromosomes) is of paramount importance to obtain desired sex of the offspring and avoid animal wastage as much as possible. However, diverse factors can affect quality of semen of different animal species especially after sex-sorting. Flow cytometry is a common method used to select male and female sperm cells and discard dead and abnormal sperm cells during the process. Thus, sperm sexing is a good advanced reproductive technology (ART) however, it is associated with the production of oxidative stress (OS) and DNA fragmentation (SDF). These findings, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting in frozen-thawed.

Seasonal Variations in Semen Quality, Testosterone Levels, and Scrotal Size following Dietary Flaxseed Oil and Ascorbic Acid in South African Indigenous Rams.

The purpose of this study was to determine the seasonal variations in semen quality, testosterone levels, and scrotal size, following dietary flaxseed oil and ascorbic acid in South African indigenous rams. A total of 22 South African indigenous rams were randomly distributed into five treatment diets from June 2021 to May 2022 (12 months). To allow for the spermatogenesis period, semen was collected after sixty days of dietary supplementation with treatment diets. Blood was collected twice a week using an 18-gauge needle and vacutainer tubes and sent to the laboratory for testosterone analysis. Semen and blood collection were repeated eight times each season. The scrotal size (circumference, length, and width) was measured using a flexible measuring tape. Data was subjected to the General Linear Model (GLM) in Minitab® 2017. Treatment means were separated using Fisher's t-test and considered significantly different when the p-value was less than 0.05. Seasons and diet had an effect on progression, total motility, and testosterone levels. For instance, NC during the spring season had the lowest progressive motility (42.84 ± 5.32), followed by the summer (49.38 ± 4.49), winter (62.46 ± 4.35), and autumn (63.26 ± 3.58). Notably, when treatment diets were introduced, improvements were realized, and there were significant differences (p < 0.05) among the seasons following supplementation of FLAX, ASCA, and FLAX + ASCA, except for FLAX in the autumn season (53.83 ± 4.16). Total motility did not differ significantly (p > 0.05) between the seasons when the NC and PC diets were supplemented; nevertheless, there was an improvement when FLAX, ASCA, and FLAX + ASCA were supplemented. Testosterone levels were significantly influenced by the seasons when negative and PC diets were supplemented. It is noteworthy that supplementing FLAX + ASCA can reverse the influence of the season on the testosterone levels (spring, 27.52 ± 4.42; summer, 20.23 ± 5.11; autumn, 25.24 ± 3.96; and winter, 25.92 ± 4.42). In conclusion, seasons do affect semen quality and testosterone levels of South African indigenous rams. However, flaxseed oil and ascorbic acid can reverse the seasonal variations in semen quality and testosterone levels.

Flaxseed Oil as a Source of Omega n-3 Fatty Acids to Improve Semen Quality from Livestock Animals: A Review.

The demand to conserve indigenous species through the cryo-gene bank is increasing. Spermatozoa remain sensitive to cryopreservation damages especially that of avian species thus limiting the use of reproductive biotechnologies such as artificial insemination in the conservation programs. Long-chain polyunsaturated fatty acid (LCPUFAs), specifically omega n-3, expanded a research interest to improve animal reproductive efficiency through improving spermatozoa quality. This is driven by the fact that mammals cannot synthesize omega-3 de-novo because they lack delta-12 and delta-15 desaturase enzymes thus supplemented in the diet is mandatory. Delta-12 and delta-15 add a double bond at the 12th and 15th carbon-carbon bond from the methyl end of fatty acids, lengthening the chain to 22 carbon molecules. Fish oil is a pioneer source of omega n-3 and n-6 fatty acids. However, there is a report that numerous fisheries are over-exploited and could collapse. Furthermore, processing techniques used for processing by-products could complement alterations of the amino acid profile and reduce protein retrieval. Alternatively, flaxseed oil contains ±52-58% of total fatty acids and lignans in the form of α-linolenic and linoleic acid. Alpha-linolenic acid (ALA,18:3n-3) is enzymatically broken-down de-novo by delta-6 desaturase and lengthened into a long-chain carbon molecule such as eicosapentaenoic acid (C20:5n-3). Nevertheless, controversial findings following the enrichment of diet with flaxseed oil have been reported. Therefore, this paper is aimed to postulate the role of flaxseed oil as an alternative source of omega n-3 and n-6 fatty acids to improve semen quality and quantity from livestock animals. These include the interaction between docosahexaenoic acid (DHA) and spermatogenesis, the interaction between docosahexaenoic acid (DHA) and testicular cells, and the effect of flaxseed oil on semen quality. It additionally assesses the antioxidants to balance the level of PUFAs in the semen.